Formation of virus-like particles from cloned cDNAs of Thogoto virus.

Functional comparison of the two gene products of Thogoto virus segment 6.

The sixth genomic segment of Thogoto virus (THOV) encodes two proteins, the viral matrix protein (M) and an accessory protein with an interferon (IFN)-antagonistic function named ML. M and ML are shown in this study to be structural components of the virion. Using an in vivo system based on the reconstitution of functional THOV ribonucleoprotein complexes from cloned cDNAs, it was demonstrated ...

Isolation of virus like particles from fecal material obtained from diarrheal calves

Plasmid-driven formation of influenza virus-like particles.

We established a plasmid-based system for generating infectious influenza virus-like particles entirely from cloned cDNAs. Human embryonic kidney cells (293T) were transfected with plasmids encoding the influenza A virus structural proteins and with a plasmid encoding an influenza virus-like viral RNA (vRNA) which contained an antisense copy of the cDNA for green fluorescence protein (GFP) flan...

Identification of identification of Hepatopancreatic Parvo-like Virus (HPV) in cultured Penaeus semisulcatus from the Islamic Republic of Iran

During the period from August 1997 to March 1998, two thousand samples of cultured Penaeus semisulcatus postlarvae and subadults were collected from five hatcheries and 20 growout farms distributed in three provinces along the costs of Persian Gulf and the Oman Sea. Based on growth signs, LM and TEM histopathology, Hepatopancreatic Parvo-like Virus (HPV) were identified from the samples. The HP...

Rescue of recombinant Thogoto virus from cloned cDNA.

Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were generated from plasmids under the control of the RNA polymerase I promoter. Using...